Figure 5.

SI-1 and SI-2 suppress replased VCaP tumor growth. A-D. Mice bearing VCaP xenografts were castrated and replased tumors were treated with vehicle control, SI-1 (25 mg/Kg i.p) or SI-2 (25mg/Kg i.p) for 3 weeks. Tumor volumes were measured twice weekly and the tumors were collected and weighed after 3 weeks of treatment. Mouse body weights were monitored twice weekly. E. Mouse blood was collected after 3 weeeks of treatment and the serum was extracted. PSA level of each group was determined by PSA ELISA kit. F. Intratumoral testosterone was assessed by ELISA on extracts from tumors. G. IHC staining of Ki67 and H/E staining in each group was performed and quantified as described in methods. * p<0.05.