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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: DNA Repair (Amst). 2020 Sep 28;96:102975. doi: 10.1016/j.dnarep.2020.102975

Fig. 3.

Fig. 3.

Isolation of cell lines pLB4-progerin and pLB4-GFP. (A) pLB4-progerin cells display nuclear fluorescence due to expression of GFP-progerin. (B) pLB4-GFP cells display cytoplasmic fluorescence due to expression of GFP. (C) Western blot of whole-cell extracts from cell lines pLB4, pLB4-progerin, and pLB4-GFP (lanes labeled 1,2,3 respectively). Samples are displayed in noncontiguous lanes from the same gel. An anti-GFP primary antibody was used. Cell line pLB4-progerin displays GFP-progerin at the expected mw of ~95 kD (lane 2) while cell line pLB4-GFP displays GFP at the expected mw of ~27 kD (lane 3).