Table 3.
Recovery of spontaneous G418-resistant segregants
| Cell line | Cells Plated (106)a | G418R Coloniesb | Median No. Coloniesc | Colony Frequency (10−7)d | HR Frequency (10−7)e |
|---|---|---|---|---|---|
| pLB4-progerin | 30 (10) | 228 | 17.5 | 76 | 34.7 |
| pLB4-GFP | 30 (10) | 20 | 2 | 6.8 | 6.8 |
| pLB4/11 | 145 (30) | 29 | 0 | 2.0 | 1.8 |
a
For each cell line, 10 or more subclones were each propagated separately to a total of 3 to 5 million cells before being plated into medium containing G418. Total number of subclones plated is presented in parentheses.
b
Total number of G418R colonies recovered from all of the subclones for each cell line.
c
Median number of colonies recovered from the subclones plated.
d
Total number of colonies recovered divided by the total number of cells plated.
e
Calculated as colony frequency multiplied by percentage of colonies characterized as bona fide HR.