Table 4.
Characterization of spontaneous G418R segregants
| Cell Line | No. Segregants Analyzed | Gene Conversionsa | HDDs | Otherb |
|---|---|---|---|---|
| pLB4-progerin | 35 | 16 | 0 | 19 |
| pLB4-GFP | 17 | 8 | 9 | 0 |
| pLB4/11 | 24 | 5 | 16 | 3 |
a
The fraction of gene conversions among HR events recovered from pLB4-progerin was significantly different from the fraction of gene conversions among HR events recovered from pLB4-GFP and pLB4/11 (p = 9.3 × 10−4 and p= 1.6 × 10−6, respectively, by two-sided Fisher exact tests).
b
The number of “other” events recovered from pLB4-progerin was significantly different from the number of such events recovered from pLB4-GFP and pLB4/11 (p= 1.1 × 10−4 and p= 1.2 × 10−3, respectively, by two-sided Fisher exact tests).