(A) Dose-response analysis of viability of K562 or GM639 cells treated for 96 hr with indicated concentrations of drug(s). Cells were incubated for 24 hr with 5-aza-dC prior to addition of PARPi. Viability assays were repeated twice, a representative experiment is shown.
(B) Effect of depletion of TP53 on the response of GM639 cells to drug treatment quantified by CellTiter-Glo viability assays. Cells were treated with 5-aza-dC, PARPi (talazoparib) or indicated combinations of PARPi and 5-aza-dC for 72 hr. Prior to drug treatment, cells were treated for 48 hr with siTP53, or non-specific siRNA, siNT2.