Figure 2. FIPs are more responsive than APCs to the activation of NFκB signaling.

a) Mural-Ikk2^CA mice were derived by breeding Pdgfrb^rtTA transgenic mice to animals expressing Cre recombinase under the control of the tetracycline-response element (TRE-Cre) and carrying the Rosa26R^Ikk2CA allele. Littermates carrying only Pdgfrb^rtTA and Rosa26R^Ikk2CA alleles (i.e. Cre⁻) were used as the control animals (Control). The addition of doxycycline (Dox) leads to activation of the Rosa26R^Ikk2CA allele and constitutively active (CA) IKK2 in Pdgfrb-expressing cells. Constitutively active IKK2 expression leads to activation of NFκB signaling.

b) mRNA levels of indicated pro-inflammatory genes in cultured FIPs and APCs 1 day following the addition of doxycycline (1μg/ml) to culture medium. Data presented were reproduced in two independent experiments. Bars represent mean ± s.e.m., * denotes p< 0.05, ** denotes p<0.01, and *** denotes p<0.001 by two-way ANOVA. Exact p values can be found in Source Data Figure 2.