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. Author manuscript; available in PMC: 2021 May 2.
Published in final edited form as: Nat Metab. 2020 Nov 2;2(11):1332–1349. doi: 10.1038/s42255-020-00301-7

Figure 6. Zfp423 overexpression in PDGFRβ+ cells attenuates WAT inflammation in obesity.

Figure 6.

a) Control (n=9; black) and Mural-Zfp423TG (n=7; red) body weights following the onset of HFD feeding at thermoneutrality.

b) WAT mass of Control and Mural-Zfp423TG mice after 1 month (n=10 for Control; n=9 for Mural-Zfp423TG) and 5 months (n=9 for Control; n=7 for Mural-Zfp423TG) of HFD feeding.

c) Frequency of total PDGFRβ+ cells, FIPs, and APCs, within gonadal WAT (gWAT) of Control (n=8) and Mural-Zfp423TG (n=12) mice after 1 month of HFD feeding.

d) Zfp423 mRNA levels within gWAT FIPs and APCs from Control (n=8) and Mural-Zfp423TG (n=12) mice after 1 month of HFD feeding.

e) Pro-inflammatory gene expression in gWAT FIPs and APCs from Control (n=8) and Mural-Zfp423TG (n=12) mice after 1 month of HFD feeding.

f) Pathway analysis of genes with differential expression (FDR q-value<0.05 and Log2 fold change>0.5) between Control and Mural-Zfp423TG FIPs after 1 month of HFD feeding.

g,h) 4x magnification images of H&E stained gWAT from Control mice maintained on HFD for 5 months.

i,j) 10x magnification images of PERILIPIN (red) and MAC-2 (green) expression in gWAT of Control mice maintained on HFD for 5 months.

k,l) 4x magnification images of H&E stained gWAT from Mural-Zfp423TG mice maintained on HFD for 5 months.

m,n) 10x magnification images of PERILIPIN (red) and MAC-2 (green) expression in gWAT of Mural-Zfp423TG mice maintained on HFD for 5 months.

o) Pro-inflammatory- and macrophage-selective gene expression in gWAT of Control (n=8) and Mural-Zfp423TG (n=8) mice after 5-months HFD feeding.

p) Cytokine protein levels in gWAT of Control (n=8) and Mural-Zfp423TG (n=8) mice after 5-months HFD feeding.

q) Frequency of total adipose tissue macrophages (ATMs) within gWAT of Control (n=8) and Mural-Zfp423TG (n=8) mice after 5 months of HFD-feeding.

r) Frequency of pro-inflammatory ATMs within gWAT of Control (n=8) and Mural-Zfp423TG (n=8) mice after 5 months of HFD-feeding.

s) Frequency of tdTomato+ macrophages within gWAT SVF of obese mice following injection of tdTomato+ monocytes. n=8 for Control and n=8 for Mural-Zfp423TG.

Bars represent mean ± s.e.m., *p< 0.05, **p<0.01 or ***p<0.001 by unpaired two-tailed Student’s t-test (o-s) or one-way ANOVA (d, e). Exact p values and numbers of repetitions can be found in Source Data Figure 6.