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. Author manuscript; available in PMC: 2021 May 2.
Published in final edited form as: Nat Metab. 2020 Nov 2;2(11):1332–1349. doi: 10.1038/s42255-020-00301-7

Extended Data Fig. 1: Thermoneutral housing amplifies the pro-inflammatory phenotype of FIPs.

Extended Data Fig. 1:

a) Experimental design: 6 weeks-old male C57BL/6 mice were transferred to thermoneutral housing conditions (TN, 30 °C) or maintained at room temperature (RT, 22 °C) for two months. Then animals were fed HFD for 3 days prior to gene expression analysis of FIPs and APCs.

b) mRNA levels of indicated genes in freshly isolated FIPs and APCs obtained following 3 days of HFD. For each experiment, n = 8 for FIPs and APCs at 22 °C. n = 4 for FIPs and APCs at 30 °C. Data presented were reproduced in two independent experiments. Bars represent mean ± s.e.m., * denotes p< 0.05, ** denotes p<0.01, and *** denotes p<0.001 by two-way ANOVA.

c) mRNA levels of TNF-signaling signature genes (see Supplementary Table 1) in freshly isolated FIPs (n=8) and APCs (n=8) obtained following 1 month of HFD at 30 °C. Bars represent mean ± s.e.m., * denotes p< 0.05, ** denotes p<0.01, and *** denotes p<0.001 by unpaired two-tailed Student’s t-test.

Exact p values and numbers of repetitions can be found in Source Data Extended Data Figure 1.