Figure 5.

Allyl sulfide photodegradable hydrogels support the expansion and repetitive passaging of intestinal organoids. a Shear rheology of swollen allyl sulfide hydrogels shows a correlation between macromer concentration and shear storage modulus. b The efficiency of colony formation from single encapsulated ISCs shows a dependence on the macromer concentration. c Allyl sulfide photodegradation was used to repetitively passage ISC colonies (p1, p2, p3), which maintained polarized epithelium, as seen by DAPI (blue) and f-actin (green) staining. Scale bars 50μm. d Relative mRNA expression for intestinal genes was similar in organoids grown in allyl sulfide photodegradable hydrogels compared to Matrigel controls. Significance determined by one-way ANOVA, N = 3, mean ± s.d., ***p < 0.001. e Colonies were formed at a slightly decreasing rate upon repetitive passaging. Open circles represent average measurements from one gel replicate, while bars represent the average of the three gel replicates.