Table 1:
A summary of the kinetic constants for the C4 minibody, scFv and their respective DFO-conjugates. The binding was assayed against the ectodomain of recombinant human PD-L1 using biolayer interferometry. The data are representative of two independent experiments. In the case of the KD and the Kdis for the C4 minibody, the constants approached the limit of detection and the instrument was not capable of reporting error calculations.
| Antibody | KD (pM) | kon (1/M*s) | Kdis (1/s) |
|---|---|---|---|
| C4 minibody | 7.39 | 2.61x105 ± 2.2x103 | 1.93x10−6 |
| C4 scFv | 545 ± 51 | 3.36x105 ± 1.9x103 | 1.83x10−4 ± 1.3x10−6 |
| DFO-minibody | 136 ± 9.5 | 1.58x105 ± 9.4x102 | 2.16x10−5 ± 1.5x10−6 |
| DFO-scFv | 541 ± 5.1 | 2.24x105 ± 2.2x103 | 2.00x10−4 ± 1.4x10−6 |