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. Author manuscript; available in PMC: 2021 Dec 1.
Published in final edited form as: Anal Biochem. 2020 Aug 4;610:113887. doi: 10.1016/j.ab.2020.113887

Figure 2: Comparison of the traditional and improved sequential purification of Torpedo californica nAChR-LFC16 complex.

Figure 2:

Chromatography was performed using a Superdex 200 10/300 increase Gel filtration GE column, flow rate of 0.25 ml/min. The elution profile was monitored at 280 nm. (a) Elution profile Tc-nAChR-LFC-16 using traditional chromatographic procedure. (b) Elution profile of the same preparation, but using sequential FPLC-Affi-Gel 15-Capto Lentil Lectin-SEC purification steps, (c) SDS-PAGE 4–20%, protein migration patterns for both purification protocols (left) traditional chromatography, (right) sequential purification, and (d and e) Microfluidic Capillary Gel Electrophoresis electropherograms showing the nAChR characteristic 2αβγδ bands for the traditional and sequential protocols.