Table 1.
Histomorphometric analysis of DMM mice with JNK deletion and wild-type (WT) controls.
| Parameter | WT Mean (SD) | JNK1−/− Mean (SD) | JNK2−/− Mean (SD) |
JNK1/2dKO MEAN (SD) |
WT vs JNK1−/− p val | WT vs JNK2−/− P VAL |
WT vs JNK1/2dKO P VAL |
|---|---|---|---|---|---|---|---|
| Art Cart Area (μm2) | 29,470 (10,860) | 20,750 (12,740) | 27,770 (8348) | 26,200 (9010) | 0.086 | 0.95 | 0.724 |
| Art Cart Thickness (μm) | 36.99 (10.54) | 42.44 (23.41) | 45.58 (8.00) | 43.24 (12.90) | 0.128 | 0.04 | 0.03 |
| Subchondral Bone Area (μm 2) | 85,070 (21,410) | 129,700 (30,170) | 98,770 (33,740) | 95,560 (41,420) | <0.0001 | 0.34 | 0.616 |
| Subchondral Bone Thickness (μm) | 84.85 (23.28) | 176.7 (40.28) | 132.6 (48.27) | 132.4 (57.79) | <0.0001 | 0.0008 | 0.004 |
| Calcified cartilage area (μm2) | 46,200 (7757) | 35,420 (6598) | 36,080 (4609) | 43,530 (8601) | 0.0005 | 0.0008 | 0.349 |
| Calcified cartilage thickness (μm) | 46.67 (8.98) | 47.85 (7.71) | 49.18 (6.96) | 61.91 (9.28) | 0.434 | 0.279 | 0.0001 |
| % Chondrocyte cell death (area cell death/art cart area) |
12.57 (6.299) | 43.06 (28.0) | 31.21 (12.66) | 38.97 (25.38) | <0.0001 | <0.0001 | 0.001 |
Histomorphometry measurements of articular cartilage (Art Cart), subchondral bone and calcified cartilage parameters in the medial tibial compartment of mid-coronal stifle sections were analyzed using the Osteomeasure Histomorphometry Program (OsteoMetrics®). WT: n=15; JNK1−/−: n=12; JNK2−/−: n=13; JNK1/2dKO n=15. Significance differences between each knockout compared to WT control was determined by Mann-Whitney test.