Table 2.
Histomorphometric analysis of aged mice with JNK deletion and wild-type (WT) controls.
| Parameter | WT Mean (SD) | JNK1−/− Mean (SD) | JNK2−/− Mean (SD) |
WT vs JNK1−/−
p-value |
WT vs JNK2−/− p-value |
|---|---|---|---|---|---|
| Art Cart Area (μm2) | 30,023 (8595) | 21,011 (19,885) | 11,295 (16,977) | 0.43 | 0.01 |
| Art Cart Thickness (μm) | 40.45 (7.194) | 28.95 (23.99) | 18.63 (20.54) | 0.38 | 0.007 |
| Subchondral Bone Area (μm 2) | 149,284 (63,334) | 155,757 (61,156) | 187,723 (68,348) | 0.65 | 0.12 |
| Subchondral Bone Thickness (μm) | 138.4 (54.51) | 159.9 (66.40) | 194.8 (77.45) | 0.36 | 0.03 |
| Calcified cartilage area (μm2) | 42,283 (7470) | 28,683 (16,730) | 23,541 (20,,481) | 0.01 | 0.01 |
| Calcified cartilage thickness (μm) | 42.11 (6.979) | 30.69 (15.24) | 26.65 (21.61) | 0.01 | 0.13 |
| % Chondrocyte cell death (area cell death/art cart area) |
56.27 (28.41) | 28.16 (30.15) | 62.82 (37.04) | 0.02 | 0.63 |
Histomorphometry measurements of articular cartilage (Art Cart), subchondral bone and calcified cartilage parameters in the medial tibial compartment of mid-coronal stifle sections were analyzed using the Osteomeasure Histomorphometry Program (OsteoMetrics®). WT: n=15; JNK1−/−: n=13; JNK2−/−: n=14. Significance differences between each knockout compared to WT control was determined by Mann Whitney test.