Characteristics of functionalized nanographenes. A. UV-vis-NIR spectra of different components, indicating the successful loading of MTX and SB onto rGO. B. Particle size analysis of rGO/MTX/SB. C. The expression of CD86 on BMDCs and secretion of TNFα from BMDCs, after the stimulation by different rGO derivatives, indicating the immune stimulation ability of rGO (n = 4, *p < 0.05). D. Cellular localization of FITC-rGO/MTX before and after laser irradiation. Bar=20 μm. E. Cytotoxic effects of different rGO derivatives on 4T1 cells at different doses, without or with irradiation of an 805-nm laser (0.75 W/cm2 for 5 min). Viability of tumor cells were analyzed by MTS assay 48 h after the treatments of rGO derivatives only, or 12 h after laser treatment with rGO derivatives, (n = 6, *P < 0.05).