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. 2020 Nov 3;8:553444. doi: 10.3389/fcell.2020.553444

FIGURE 5.

FIGURE 5

Comparing MSC-EV production in bioreactor and static culture systems, using MSC from different sources. (A) EV concentration (particles/mL) in the cell culture conditioned medium from BM, AT, and UCM MSC cultures in static and Vertical-WheelTM bioreactor systems. MSC from three different donors were used for each tissue source (i.e., n = 3 biological replicates). Results are presented as mean ± SEM (n = 3). Upper-right panel: Summarized paired analysis comparing EV concentration in static and Vertical-WheelTM bioreactor systems, for each MSC donor. Paired statistical analysis (paired t-test ∗∗P = 0.0027) (n = 9). (B) Specific EV concentration (particles/cell) in the cell culture conditioned medium from BM, AT, and UCM MSC cultures in static and Vertical-WheelTM bioreactor systems. MSC from three different donors were used for each tissue source. In static cultures, each T-175 yielded 1.2 – 6.6 × 106 cells upon 4 – 9 days of expansion, regardless of the cell tissue source. Results are presented as mean ± SEM (n = 3; n = 2 for UCM-static). Upper-right panel: Summarized paired analysis comparing specific EV concentration in static and Vertical-WheelTM bioreactor systems, for each MSC donor. (C) Particle to protein ratios (PPR) (particle/μg protein) of EV samples obtained from BM, AT and UCM MSC, cultured in static and Vertical-WheelTM bioreactor systems. MSC from three different donors were used for each tissue source. Results are presented as mean ± SEM (n = 3). Upper-right panel: Violin plot of PPR of MSC-EV samples obtained in static and Vertical-WheelTM bioreactor systems.