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. 2020 Feb 17;59(8):3669–3689. doi: 10.1007/s00394-020-02200-9

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Effect of B. coagulans spores, GBRS and synbiotic on goblet cells. The paraffin embedded sections were stained with Alcian Blue to detect changes in goblet cells and in production of mucus in distal colonic tissue in each experimental group (× 40). Staining intensity (IOD) of respective group is illustrated in the graph. Results expressed as mean ± SEM (n = 4 per group), evaluated by one-way ANOVA followed by Tukey’s test (*p < 0.05, ***p < 0.001)