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. 2020 Nov 3;11:572419. doi: 10.3389/fmicb.2020.572419

Figure 2.

Figure 2

Dominant negative effect of CTD on RelA synthetic activity in vitro. (p)ppGpp production by RelA in vitro. (A) Solid circles (●) WT-RelA in the presence of ribosomes; empty circles (o) – WT-RelA in the absence of ribosomes; solid squares (▪) – Rel-C638F in the presence of ribosomes; empty squares (▫) – Rel-C638F in the absence of ribosomes. (B,C) (p)ppGpp production by 1 μg of RelA with the addition of increasing amounts of (B) YG4 and (C) YG4-C638F. (D,E) (p)ppGpp production by 1 μg of Rel-C638F with the addition of increasing amounts of (D) YG4 and (E) Rel251.