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RelA binding to the ribosomes. Western blot analysis of the ribosomal fraction from an in vitro reaction of (p)ppGpp synthesis by 1 μg of RelA, with the addition of increasing amounts of (A). YG4 and (B) YG4-C638F as indicated in (C) and (D), respectively. (C,D) Quantification of RelA bound to the ribosome from (A) and (B), respectively.
