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. 2020 Nov 16;10:19882. doi: 10.1038/s41598-020-76322-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of cell phenotype for the hinge area of second-generation TEHVs. Immunofluorescence images at the hinge area stained for M1 (green) and M2 (red) macrophages at 8 weeks (A) and 52 weeks (B) follow-up. (C) Total cell number quantification in the wall and hinge region of second-generation TEHVs. (D) Quantification of M1 and M2 macrophages in the hinge region at the different time-points considered shows a mild prevalence of M1 over M2 positive cells. For immunofluorescence pictures: scale bars: 100 μm, zoom 1 × . For every measurement, cell number is normalized per μm³.