Table 1.
p53 CTD phosphopeptides affinity to 14-3-3 isoforms as determined by FP assay.
p53 CTD peptides | Kd (μM)a | |||
---|---|---|---|---|
γ | ε | τ | σ | |
pS366 | 17 ± 2 | 16 ± 2 | 28 ± 3 | 24 ± 4 |
pS378 | 20 ± 2 | 18 ± 3 | 27 ± 3 | 22 ± 3 |
pT387 | 14 ± 3 | 11 ± 2 | 24 ± 4 | 23 ± 3 |
pS366/pT387 | 0.14 ± 0.07 | 0.18 ± 0.05 | 6.2 ± 0.3 | 3.5 ± 0.4 |
pS366 /pS378 | 0.48 ± 0.05 | 0.51 ± 0.03 | 2.1 ± 0.4 | 2.2 ± 0.3 |
pS378/pT387 | 0.45 ± 0.03 | 0.80 ± 0.06 | 3.3 ± 0.2 | 7.3 ± 0.3 |
wt (362–393) | n.q. | n.q. | n.q. | n.q. |
n.q. not quantifiable.
aValues taken from published data16