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. 2020 Nov 16;10:19876. doi: 10.1038/s41598-020-76428-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cell type-specific EGFP protein expression in in sEGFP and P2X7-EGFP BAC transgenic P2X7 reporter mice. Brain slices from BAC transgenic mice were co-labelled with an anti-GFP antibody and antibodies against marker proteins for microglia (Iba1), neurons (NeuN), and oligodendrocytes (Olig2). Representative results are shown for the CA3-CA2 border (A), the dentate gyrus (B) and cerebellum (C). DAPI was used as nuclear counter stain (blue). Arrowheads indicate cells with neuron-like morphology in the CA regions. SLu, stratum lucidum; CA, cornu ammonis; DG, dentate gyrus; ML, molecular layer; GL, granular layer. Scale bar: 50 μm.