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. 2020 Nov 16;10:19876. doi: 10.1038/s41598-020-76428-0

Figure 7.

Figure 7

Functional phenotype of the sEGFP reporter mouse. (A) Experimental design of the intra-amygdala mouse model of status epilepticus. Status epilepticus is induced by an injection of 0.2 µg kainic acid (KA) into the basolateral nucleus of the ipsilateral amygdala. To reduce mortality and morbidity, the anti-convulsant lorazepam (Lz) is administered 40 min following KA injection. (B) Representative EEG recordings presented as heat maps of frequency and amplitude data during status epilepticus of wt and sEGFP mice. (C) Graphs showing similar seizure severity between wt and sEGFP mice during a 40 min recording period starting at the time of intraamygdala KA injection until administration of anticonvulsant lorazepam (wt, n = 3 and sEGFP, n = 4). (D) Graph showing similar changes in behaviour based on the Racine scale between genotypes (wt, n = 3 and sEGFP, n = 4). (E) Graph and photomicrographs (10 × lens) showing less FjB-positive cells in the ipsilateral CA3 subfield of the hippocampus 24 h after status epilepticus in sEGFP mice compared with wt mice (n = 3 (wt), 63.00 ± 4.359; n = 4 (sEGFP), 38.00 ± 6.377; t = 2.983 df = 5, unpaired t-test; p = 0.031). Scale bar, 100 μm.