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. 2020 Nov 16;11:5823. doi: 10.1038/s41467-020-19452-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schematic of experimental approach. b Schematic representation of how nanopore sequencing facilitates de novo amplicon assembly and can be used to simultaneously to detect regulatory elements through DNA methylation analysis. c Composite DNA methylation signal detected using nanopore sequencing over genes expressed at high (HighExpr) vs. low levels (LowExpr). d Motif analysis based on accessibility in regulatory elements co-amplified on MYCN amplicons (unadjusted P values from one-sided binomial test against nucleotide composition-matched background sequences). e Amplicon-specific methylation pattern detected in three neuroblastoma cell lines (Kelly, IMR-5/75, CHP-212) using nanopore sequencing-based DNA methylation analysis.