Figure 4.

Cell viability and apoptosis of miR-M2-5p-overexpressed (A,B) or GaHV-2-infected (C,D) CEF cells. At different time points post miRNA transfection or virus infection, the CEF cells were harvested for Annexin V-FITC and propidium iodide (PI) staining for apoptosis analysis using flow cytometry. CCK-8 assay was used to assess cell viability. Hpt, hours post-transfection; Hpi, hours post-infection. CEFs without transfection or infection serve as mock controls. Data are shown as mean ± SD of three independent experiments. Independent sample t-test was used to analyze the statistical differences between groups. Values of p indicated on columns were used for statistical analyses; ns, no significance.