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. 2020 Nov 3;11:596422. doi: 10.3389/fmicb.2020.596422

Figure 6.

Figure 6

The MYOD1-mediated IGF2 gene expressions and cell viability. (A,B) mRNA and protein expression levels of MYOD1 in CEFs overexpressing distinct siRNAs. (C) Proliferation analysis of MYOD1-silenced CEF cells. CEF cells transfected with MYOD1 siRNAs were harvested at 48 hpt for CCK-8 assay. (D,E) RT-qPCR and western blot analysis of gga-miR-223 and IGF2 expressions in MYOD1-silenced CEF cells at 48 hpt. (F,G) Expression levels of gga-miR-223, MYOD1, and IGF2 proteins in CEFs overexpressing miR-M2-5p determined by RT-qPCR or western blot analysis. (H) Relative signal intensities in western blot analysis of the MYOD1 and IGF2 proteins in CEFs overexpressing miR-M2-5p. (I,J) Expression levels of gga-miR-223, MYOD1, and IGF2 proteins in GaHV-2-infected CEFs determined by RT-qPCR or western blot analysis. (K) Relative signal intensities in western blot analysis of the MYOD1 and IGF2 proteins in GaHV-2-infected CEFs. Numbers were normalized to the corresponding signal from the β-actin bands. Error bars are derived from three independent replicates. Values of p indicated on columns were used for statistical analyses; ns, no significance.