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. 2020 Nov 3;11:596422. doi: 10.3389/fmicb.2020.596422

Figure 7.

Figure 7

Cell apoptosis and gene expressions involved in miR-M2-5p-mediated MYOD1/Caspase-3 signaling pathway. (A) Apoptosis analysis of the MYOD1-silenced CEF cells. CEF cells transfected with MYOD1 siRNA were harvested at 48 hpt for Annexin V-FITC and propidium iodide (PI) staining for apoptosis analysis using flow cytometry. Data are shown as mean ± SD of three independent experiments. (B) RT-qPCR analysis of gga-miR-1 and gga-miR-206 expressions in the MYOD1-silenced CEF cells at 48 hpt. (C) Western blot analysis of proteins associated with the MYOD1/Caspase3 signaling pathway in the MYOD1-silenced CEF cells at 48 hpt. (D) Expressions of gga-miR-1 and gga-miR-206 in CEFs overexpressing miR-M2-5p. (E) Expressions of proteins associated with the MYOD1/Caspase3 signaling pathway in CEFs overexpressing miR-M2-5p. (F) Relative signal intensities in western blot analysis of the proteins associated with the MYOD1/Caspase3 signaling pathway in CEFs overexpressing miR-M2-5p. (G) Expressions of gga-miR-1 and gga-miR-206 in GaHV-2-infected CEFs. (H) Expressions of proteins associated with the MYOD1/Caspase3 signaling pathway in GaHV-2-infected CEFs. (I) Relative signal intensities in western blot analysis of the proteins associated with the MYOD1/Caspase3 signaling pathway in GaHV-2-infected CEFs. Numbers were normalized to the corresponding signal from the β-actin bands. Error bars are derived from three independent replicates. Values of p indicated on columns were used for statistical analyses; ns, no significance.