Table 3.
Direct sequencing and PCR group—combined statistics
| Direct sequencing group | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Study | Direct method | Total PTCs | TP | TN | FP | FN | Sensitivity | Specificity | Accuracy | PPV | NPV |
| Bullock (2012) | Direct (Sanger) | 96 | 59 | 28 | 9 | 0 | 100.00% | 75.68% | 90.63% | 86.76% | 100.00% |
| Capper (2011) | Direct (Sanger) | ||||||||||
| Dvorak (2014) | Direct (Sanger) | 73 | 52 | 19 | 2 | 0 | 100.00% | 90.48% | 97.26% | 96.30% | 100.00% |
| Ghossein (2013) | Mass spectrometry | 31 | 15 | 16 | 0 | 0 | 100.00% | 100.00% | 100.00% | 100.00% | 100.00% |
| Fisher (2013) | Pyro | 25 | 10 | 11 | 4 | 0 | 100.00% | 73.33% | 84.00% | 71.43% | 100.00% |
| Kim (2014) | Direct (Sanger) | 91 | 56 | 16 | 12 | 7 | 88.89% | 57.14% | 79.12% | 82.35% | 69.57% |
| Kim (2014) | Pyro Seq | 91 | 56 | 14 | 12 | 9 | 86.15% | 53.85% | 76.92% | 82.35% | 60.87% |
| Kim (2018) | Direct (Sanger) | 697 | 581 | 70 | 46 | 0 | 100.00% | 60.34% | 93.40% | 92.66% | 100.00% |
| Koperek (2012) | Direct (Sanger) | ||||||||||
| Loo (2018) | Direct (Sanger) | 12 | 5 | 7 | 0 | 0 | 100.00% | 100.00% | 100.00% | 100.00% | 100.00% |
| Martinuzzi (2016) | Direct (Sanger) | 85 | 54 | 23 | 8 | 0 | 100.00% | 74.19% | 90.59% | 87.10% | 100.00% |
| Oh (2018) | Direct (Sanger) | 23 | 16 | 5 | 2 | 0 | 100.00% | 71.43% | 91.30% | 88.89% | 100.00% |
| Qiu (2015) | Direct (Sanger) | 127 | 100 | 25 | 2 | 0 | 100.00% | 92.59% | 98.43% | 98.04% | 100.00% |
| ZagZag (2013) | Direct (Sanger) | 37 | 25 | 9 | 0 | 3 | 89.29% | 100.00% | 91.89% | 100.00% | 75.00% |
| Zhao (2019) | Direct (Sanger) | 185 | 140 | 43 | 1 | 1 | 99.29% | 97.73% | 98.92% | 99.29% | 97.73% |
| Zhu (2016) | Direct (Sanger) | 118 | 73 | 37 | 8 | 0 | 100.00% | 82.22% | 93.22% | 90.12% | 100.00% |
| PCR based group | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Study | PCR method | Total PTCs | TP | TN | FP | FN | Sensitivity | Specificity | Accuracy | PPV | NPV |
| Chen (2018) | quant-rtPCR | 40 | 34 | 4 | 2 | 0 | 100.00% | 66.67% | 95.00% | 94.44% | 100.00% |
| de Biase (2014) | ASLNA-qPCR | 20 | 18 | 0 | 0 | 2 | 90.00% | 90.00% | 100.00% | 0.00% | |
| Elmageed (2017) | rt-PCR | 130 | 97 | 28 | 2 | 3 | 97.00% | 93.33% | 96.15% | 97.98% | 90.32% |
| Ilie (2014) | SNaPshot | ||||||||||
| Jung (2015) | PCR | 467 | 392 | 65 | 9 | 1 | 99.75% | 87.84% | 97.86% | 97.76% | 98.48% |
| Kim (2014) | PNA clamp | 91 | 56 | 17 | 12 | 6 | 90.32% | 58.62% | 80.22% | 82.35% | 73.91% |
| Kim (2014) | rt-PCR | 91 | 58 | 15 | 10 | 8 | 87.88% | 60.00% | 80.22% | 85.29% | 65.22% |
| Martinuzzi (2016) | PNA-Clamp | 85 | 58 | 21 | 2 | 4 | 93.55% | 91.30% | 92.94% | 96.67% | 84.00% |
| McKelvie (2013) | C-PCR (SNaPshot) | 71 | 49 | 21 | 1 | 0 | 100.00% | 95.45% | 98.59% | 98.00% | 100.00% |
| Na (2015) | rt-PCR | 104 | 71 | 29 | 4 | 0 | 100.00% | 87.88% | 96.15% | 94.67% | 100.00% |
| PajaFano (2017) | rt-PCR | ||||||||||
| Qiu (2015) | rt-PCR | 127 | 100 | 25 | 2 | 0 | 100.00% | 92.59% | 98.43% | 98.04% | 100.00% |
| Routhier (2013) | SNaPshot | 15 | 9 | 6 | 0 | 0 | 100.00% | 100.00% | 100.00% | 100.00% | 100.00% |
| Sun (2015) | rt-PCR | 556 | 414 | 137 | 0 | 5 | 98.81% | 100.00% | 99.10% | 100.00% | 96.48% |
| Szymonek (2017) | rt-PCR#1 | 137 | 69 | 46 | 9 | 13 | 84.15% | 83.64% | 83.94% | 88.46% | 77.97% |
| rt-PCR#2 | 137 | 80 | 49 | 6 | 2 | 97.56% | 89.09% | 94.16% | 93.02% | 96.08% | |
| Zhang (2018) | ARMS-PCR | 132 | 106 | 21 | 5 | 0 | 100.00% | 80.77% | 96.21% | 95.50% | 100.00% |
| Zhao (2019) | PCR | 185 | 142 | 35 | 4 | 4 | 97.26% | 89.74% | 95.68% | 97.26% | 89.74% |