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. 2020 Nov 3;8:568116. doi: 10.3389/fcell.2020.568116

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Copyright © 2020 Liu, Liu, Pan, Zhang, Qin, Chen, Li, Chen, Zheng, Liu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of CR pretreatment on myocardial ischemic injury. (A) Representative morphological images of myocardial tissue in norm, MI, and CR + MI groups after TTC staining. (B) TUNEL assay of myocardial tissues in norm, MI, and CR + MI groups. Brown staining of the nucleus indicates apoptosis. (C) The activities of serum CK were determined by CK assay kit in norm, MI, and CR + MI groups. (D) The activities of serum LDH were examined by LDH activity detection kit in norm, MI, and CR + MI groups. (E) The levels of serum CK-MB1 were measured by CK-MB1 ELISA kit in norm, MI, and CR + MI groups. The data are presented as the mean ± SEM (n = 6). *P < 0.05, **P < 0.01 compared with the MI group.