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. 2020 Nov 3;8:568116. doi: 10.3389/fcell.2020.568116

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Copyright © 2020 Liu, Liu, Pan, Zhang, Qin, Chen, Li, Chen, Zheng, Liu and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Target genes of CR-related tsRNAs and their verification. (A) Venn diagram showing 982 mRNA targets of five CR pretreatment–related tsRNAs that were predicted by two prediction algorithms. (B) The target of each tsRNA is shown separately. (C) The binding region and seed sequence of five randomly selected mRNA transcripts (each corresponding to a tsRNA sequence). (D) Verification of target predictions of Mastl and Gpx8 via qRT-PCR. (E) Verification of target predictions of Casp2, Rbfox1, and Stk39 via qRT-PCR. The data are presented as the mean ± SEM (n = 6). *P < 0.05, **P < 0.01 compared with the MI group.