Fig. 2.

CXCR5 is upregulated in the motor neurons of mSOD1 mice during the disease progression. (a) Real-time PCR for CXCL13 transcript in the lumbar spinal cord of 129Sv-mSOD1 mice. Data are normalised to β-actin and expressed as the mean ± SEM fold-change ratio between 129Sv-mSOD1 mice compared to respective age-matched Ntg littermates. *P < 0.05; ^⁎⁎P < 0.01 (Vs. Ntg) by one-way ANOVA with Tukey's post-analysis. (b) Representative co-localization of CXCR5 (green) with NT (purple) but not with GFAP (red) in the ventral horn of the spinal cord of fast-progressing mice; scale bar: 20 μm. (c) Confocal representative images showing the progressive increase in CXCR5 staining in the lumbar spinal cord of 129Sv-mSOD1 mice.