Fig. 9.

Cell adhesion on three samples after incubating for 24 h. (a) Cell morphology and their filopodia observed by FE-SEM. The insets show the morphology of a single cell to compare the cell areas. The red triangles show the small lateral protrusions from the filopodia. (b) Confocal mosaic images of cell distributions on entire samples before and after being centrifuged at 600 rpm or 1300 rpm for 5 min. And the related cell counts (c) and cell adhesion forces (d) were also calculated. (e) The represent staining images of F-actin (green) and vinculin (red) on all samples. Black scale bar = 200 nm, white scale bar = 50 μm, yellow bar = 2 mm *p < 0.05, **p < 0.01, ***p < 0.001(VS TNT, PMSTF). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)