Table 2.
Accumulation of fluoroquinolones (FQs) in two strains, BW25113 (WT) and BW25113Δtolc (ΔC) after correction for wash. Mtw and Mpw are accumulation numbers in the whole cell and periplasm and cytoplasm, respectively. Cpw and Ctw refer to the concentration of drug in the periplasm and whole cell respectively. Accumulated fluoroquinolones were at higher concentrations than the external media.
| FQs/strain | Mw (ng) | Mpw (ng) | Mtw (ng) | Cpw (μg/ml) | Ctw (μg/ml) |
|---|---|---|---|---|---|
| Ciprofloxacin ΔC | 40.4 ± 7.3 | 41.0 ± 7.9 | 142.6 ± 20.1 | 25.6 ± 5.0 | 8.9 ± 1.2 |
| Ciprofloxacin WT | 37.0 ± 5.6 | 17.3 ± 5.7 | 86.1 ± 8.8 | 10.9 ± 3.5 | 5.3 ± 0.5 |
| Norfloxacin ΔC | 32.4 ± 1.1 | 35.6 ± 4.3 | 148.4 ± 2.2 | 22.3 ± 2.7 | 9.2 ± 0.1 |
| Norfloxacin WT | 32.6 ± 1.4 | 21.8 ± 2.1 | 115.0 ± 4.9 | 13.6 ± 1.3 | 7.1 ± 0.3 |
| Enrofloxacin ΔC | 37.9 ± 3.9 | 90.7 ± 3.9 | 149.5 ± 17.0 | 56.7 ± 2.4 | 9.3 ± 1.1 |
| Enrofloxacin WT | 20.2 ± 3.4 | 2.7 ± 3.5 | 22.8 ± 5.0 | 1.7 ± 2.2 | 1.4 ± 0.3 |
| Levofloxacin ΔC | 42.6 ± 0.6 | 29.6 ± 1.7 | 74.9 ± 10.5 | 18.5 ± 1.1 | 4.7 ± 0.6 |
| Levofloxacin WT | 36.2 ± 3.1 | 2.6 ± 3.7 | 16.5 ± 3.9 | 1.6 ± 2.3 | 1.0 ± 0.2 |
| Lomefloxacin ΔC | 21.0 ± 5.5 | 70.1 ± 6.0 | 128.0 ± 12.4 | 43.8 ± 3.7 | 8.0 ± 0.8 |
| Lomefloxacin WT | 21.6 ± 5.9 | 11.9 ± 6.6 | 15.4 ± 8.7 | 7.4 ± 4.2 | 1.0 ± 0.5 |
| Ofloxacin ΔC | 40.1 ± 5.0 | 30.3 ± 8.0 | 56.4 ± 6.8 | 19.0 ± 5.0 | 3.5 ± 0.4 |
| Ofloxacin WT | 25.7 ± 0.7 | 3.2 ± 2.2 | 9.7 ± 3.2 | 2.0 ± 1.4 | 0.6 ± 0.2 |
| Fleroxacin ΔC | 65.4 ± 6.4 | 34.9 ± 7.3 | 81.0 ± 8.1 | 21.8 ± 4.5 | 5.0 ± 0.5 |
| Fleroxacin WT | 55.8 ± 2.0 | 2.6 ± 2.5 | 17.6 ± 5.0 | 1.7 ± 1.6 | 1.1 ± 0.3 |
| Marbofloxacin ΔC | 22.3 ± 3.5 | 54.4 ± 5.9 | 78.1 ± 5.2 | 34.0 ± 3.7 | 4.9 ± 0.3 |
| Marbofloxacin WT | 12.0 ± 1.6 | 27.6 ± 1.9 | 34.8 ± 5.8 | 17.2 ± 1.2 | 2.2 ± 0.4 |
| Moxifloxacin ΔC | 37.7 ± 4.3 | 30.3 ± 5.0 | 86.5 ± 4.3 | 19.0 ± 3.1 | 5.4 ± 0.3 |
| Moxifloxacin WT | 10.1 ± 2.1 | 3.9 ± 2.2 | 17.4 ± 2.3 | 2.4 ± 1.4 | 1.1 ± 0.1 |