Fig. 3.

Coarse-grained (CG) simulation model for the pre-fusion of vesicle initiated by Slp4-a. The model contains ~77,679 atoms with an overall dimension of 160 × 175 × 300 Å³. The center of mass of the whole protein system is located at the center of the model system. CG waters are shown in bright blue color, while CG lipids are presented in gray color. The initial distance between the two membranes is 55 Å. NaCl is added as a neutralizer for the system and Ca²⁺ is added in a smaller concentration than NaCl. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)