Figure 2.

Validation of HyperLOPIT-Predicted Subcellular Locations

(A) Examples of uncharacterized proteins epitope tagged and detected by immunofluorescence microscopy (magenta) co-located with named marker proteins (green). Cell outlines are indicated (dashed lines). See Figure S2 for all validated proteins. Scale bar, 10 μm.

(B) Optical super-resolution (3D-SIM) images of select proteins (magenta) from (A) with subcellular marker proteins (green). Arrows indicate the cell posterior-to-anterior cell axis. Scale bar, 1 μm.