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Optimized FACS instrument parameters for 30–50 μm double emulsion droplet analysis and high-recovery sorting
| Instrument parameter | Aria II (BD) | SH800 (Sony) |
|---|---|---|
| Nozzle size | 130 μm | 130 μm |
| ND filter | 1.5 ND | – |
| Trigger | FSC | FSC |
| Threshold | 1200 | 0.67% |
| FSC gain | 25 | 1 |
| SSC (BSC) gain | 170 | 28% |
| FITC gain | 439 | 39% |
| APC/A647 gain | 500 | 40% |
| Sample pressure | 3 flow rate | 9 psi |
| System pressure | 10 psi | 9 psi |
| Agitation | 300 rpm | High |
| Drop frequency | 14.5 kHz | 12 kHz |
