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. Author manuscript; available in PMC: 2020 Nov 17.
Published in final edited form as: Leukemia. 2014 Feb 28;28(10):2016–2027. doi: 10.1038/leu.2014.92

Figure 6.

Figure 6.

Comparison of resting, activated and nonsuppressive fractions of Treg. (a) Gated CD4+ T cells were analyzed according to Miyara and Sakaguchi to assess the frequency of fraction I ‘resting’ (CD45RA+, FoxP3lo), fraction II ‘activated’ (CD45RA−, FoxP3hi) and fraction III ‘nonsuppressive’ (CD45RA−, FoxP3lo) Treg subsets. (b–i) Graphs of the frequency of expression of markers of Treg functional activity in the three Miyara fractions in each of the 10 lichenoid (b–e) and 10 sclerotic (f–i) cGVHD patients: (b, f) CTLA4, (c, g) CD39, (d, h) ICOS and (e, i) Ki-67. (j, k) Graphs comparing Treg populations in patients with severe lichenoid oral or cutaneous cGVHD (Lich; n = 10; white), severe sclerotic skin (Scl; n = 6; light gray); cGVHD patients from the NIH natural history cohort (cGVHD; n = 47; medium gray) and normal healthy controls (ND; n = 29; black). (f) Graph of the percentages of FoxP3+ Treg within CD3+ CD4+ T cells in peripheral blood. (g) Graph of the proportions of FoxP3+ Treg cells in each of the three Miyara fractions in these patients. In all box and whisker plots, medians are indicated by horizontal bars, quartiles by box outline and minimum and maximum values by whiskers. Mann–Whitney comparisons with P-values <0.05 are indicated by *, <0.005 as ** and <0.0005 as ***. All tests were two sided. NS, nonsignificant.