Table 1.

Effect of PTL060 on Body Mass and Plasma Lipids in ApoE−/− Mice

Prevention Experiments	Aortic Tx Recipientsa—Fed HFD 6 to 14 wk (n=6 per group)						Single Injectiona—Fed HFD 6 to 12 wk (n=6 per group)
	BL/6	CD31‐TFPI‐Tg	P Valueb	BL/6	CD31‐Hir‐Tg	P Valueb	PBS	HLL (5 μg/g)	PTL060 (2.5 μg/g)	PTL060 (5 μg/g)	PTL060 (10 μg/g)	P Valuec
Body mass
Age 6 wk	18.6±0.27	18.6±0.25	0.99	19.6±0.42	19.6±0.31	0.99	19.0±0.40	19.4±0.22	19.4±0.44	19.4±0.38	19.2±0.24	0.87
End of experiment	25.2±1.71	24.6±1.36	0.63	26.8±0.34	25.9±0.77	0.99	32.0±1.63	32.0±0.99	31.4±0.84	29.8±0.35	29.4±0.73	0.36
Cholesterol, mmol/L	53.1±3.31	52.9±3.67	0.99	50.1±4.98	50.2±5.77	0.99	51.0±5.56	54.1±5.70	49.3±4.56	50.3±4.36	50.1±5.44	0.97
Triglycerides, mmol/)	2.1±0.14	2.0±0.20	0.99	2.0±0.13	2.0±0.14	0.99	2.1±0.31	2.1±0.36	2.2±0.28	2.2±0.27	2.1±0.30	1.00
HDL, mmol/L	1.5±0.11	1.5±0.10	0.99	1.6±0.07	1.5±0.08	0.99	1.4±0.28	1.5±0.08	1.6±0.12	1.5±0.09	1.5±0.09	0.92
LDL, mmol/L	57.2±2.73	55.2±3.49	0.97	63.9±9.97	61.0±6.28	0.92	55.6±6.64	54.7±4.48	54.1±4.72	51.7±5.89	52.8±5.42	0.99

Regression Experiments	6‐wk‐old	Series 1 HFD 6 to 28 wks (n=6 per group)					Series 2 HFD 6 to 28 wk (n=6 per group)
		Baselined	PBSe	Tail Only	PTL060 (10 μg/g)	P Valuec	Baselined	PBS	HLL (5 μg/g)	PTL060 (5 μg/g)	PTL060 (10 μg/g)	P Valuec
Body mass
Age 6 wk	···	19.3±0.28	19.6±0.37	19.1±0.15	19.3±0.38	0.74	20.2±0.39	20.1±0.38	20.6±0.07	20.1±0.30	20.3±0.26	0.73
Age 22 wk	···	30.5±0.58	32.8±0.44	30.4±0.90	32.0±0.81	0.19	31.5±1.34	31.4±0.84	31.1±0.66	31.2±1.00	31.9±0.78	0.85
End of experiment	···	···	33.2±0.31	31.8±0.73	28.7±1.55	0.03	···	32.9±1.19	31.4±0.33	30.2±0.93	29.8±0.59	0.11
Cholesterol, mmol/L	10.3±4.6	56.7±6.08	61.5±6.51	61.9±6.35	57.9±3.61	0.88	54.9±6.25	56.9±6.93	54.1±5.39	53.9±6.31	54.3±13.1	0.41
Triglycerides, mmol/L	0.36±0.14	2.08±0.15	2.25±0.11	2.49±0.13	2.19±0.16	0.26	2.27±0.28	1.68±0.31	2.21±0.38	2.2±0.33	2.16±0.69	0.86
HDL, mmol/L	4.46±1.1	1.54±0.007	1.49±0.06	1.46±0.06	1.56±0.06	0.65	1.61±0.12	1.58±0.11	1.78±0.2	1.6±0.16	1.68±0.38	0.27
LDL, mmol/L	14.4±2.8	46.5±3.39	56.1±2.92	57.4±2.57	50.8±2.84	0.07	53.8±3.51	62.7±4.43	63.5±3.55	60.6±6.79	63.3±16.7	0.97

“n” refers to number of animals per group. Samples from each animal were analyzed in triplicate. BL/6 indicates C57BL/6J; HDL, high‐density lipoprotein; HFD, high‐fat diet; HLL, hirulo analogue chemically modified to accept the myristoyl tail (Please note: HLL 5 μg is equimolar to PTL060 10 μg); LDL, low‐density lipoprotein; and TFPI, tissue factor pathway inhibitor.

^aIn prevention experiments, aortic transplants performed, and single injections given to mice aged 8 weeks, 2 weeks after starting a high‐fat diet.

^bTwo‐way ANOVA for multiple groups.

^cOne‐way ANOVA for multiple groups (NB: values from 6‐week‐old mice not included in comparisons).

^dBaseline=week 22. Mice in the “baseline” groups were harvested at this timepoint before any treatment.

^eTreatments given weekly by intravenous injection for 6 weeks (mice aged 22–28 weeks).