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. 2020 Jun 17;9(13):e015261. doi: 10.1161/JAHA.119.015261

Figure 3. αSMA and RAGE were colocalized, part of which were costained with ALP, osteocalcin, and SMemb in calcified aortic stenosis valves.

Figure 3

A and B, Immunofluorescence imaging of αSMA and RAGE in aortic stenosis patients (n=6) and controls (n=2). C, Immunofluorescent staining of ALP (n=5), osteocalcin (n=5), and SMemb (n=2) in aortic stenosis valves. TOPRO‐3 was used for nuclear staining. αSMA indicates α–smooth muscle actin; ALP, alkaline phosphatase; RAGE, receptor for advanced glycation end products; and SMemb, nonmuscle myosin heavy chain.