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. 2020 Nov 17;39:246. doi: 10.1186/s13046-020-01758-w

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — MTMR3 is a direct target of miR-99a-5p. a Potential binding site of miR-99a-5p for MTMR3 as predicted by TargetScan. b Luciferase reporter assay for determining whether miR-99a-5p could bind to wt-MTMR3–3’UTR or mut-MTMR3–3’UTR. c Pull-down assay confirmation of MTMR3 as a target of miR-99a-5p. d RIP assay confirmation of the interaction between miR-99a-5p and MTMR3. e qRT-PCR analysis revealing that downregulation of miR-99a-5p could increase the expression levels of MTMR3. f Increased protein expression levels of MTMR3 after knockdown of miR-99a-5p. g qRT-PCR analysis of the expression of MTMR3 CDDP-sensitive and CDDP-resistant GC cells. h MTMR3 detection in CDDP-resistant and CDDP-sensitive GC cells by western blotting. i MTMR3 expression detection using qRT-PCR in CDDP-sensitive and CDDP-resistant GC tissues. j Negative correlation between MTMR3 expression and miR-99a-5p expression. k and l qRT-PCR analysis showing that MTMR3 expression was increased by circMCTP2 but reversed by overexpression of miR-99a-5p. m and n Diminishment of the promotive effect of circMCTP2 on the protein expression of MTMR3 by the overexpression of miR-99a-5p. (*p < 0.05, **p < 0.01. Data are expressed as the means ± SDs)