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In vitro genome ejection efficiency decreases with proteinase K treated OmpA-TMS.flex. The “% remaining virions” was calculated as the number of PFUs remaining after incubation with S. flexneri LPS and OmpA-TMS.flex (untreated and proteinase K treated) divided by the number of PFUs when incubated with only buffer. Each data point is an average of at least three separate experiments; error bars signify one standard deviation.
