Table 1.

Cell lines derived from different hosts were tested for the susceptibility to SADS-CoV.

Host	Cell line	Origin of cell line	Expression of N protein at 24 hpi^a	Cytopathic effect at 72hpi^b
Bat	RaLu^#	Rhinolophus. affinis lung	++++	N
	RsHe	Rhinolophus. sinicus heart	+	N
	RlKi	Rousettus leschenaultia kidney	++++	N
	PaKi	Pteropus alecto kidney	++++	N
Swine	SIEC	Sus scrofa intestine	+	N/D
	ST*	S. scrofa testicle	++++	N
	IBRS	S. scrofa kidney	++	P
	LLC-PK1*	S. scrofa kidney	+	N
Human	A549*	Homo sapiens lung	++	N
	HeLa*	H. sapiens cervix	+	P
	Hep2	H. sapiens larynx	++	N
	RD	H. sapiens embryonic	++++	N
Monkey	Vero*	Chlorocebus aethiops kidney	++++	P
Monkey	LLC-MK2	Macaca mulatta kidney	+++	N
Cat	FK	Felinae kidney	++	N/D
Dog	MDCK*	Canis familiaris kidney	+	N
Mink	Mv.1.Lu	Mustla vison lung	++++	N
Hamster	BHK-21*	Mesocricetus auratus kidney	++	N
Rat	BRL*	Rattus norvegicus liver	+	N
Mouse	L929	Mus musculus lung	+	N

The susceptibility of SADS-CoV in trypsin-treated cells was determined by expression of SADS-CoV N protein and Cytopathic effect.

^aExpression efficiency of N protein is defined by green fluorescence positive cell ratio; + , 1%–5%; ++ , > 5%–10%; +++ , > 10%–25%; ++++ , > 25%.

^bP and N are defined as positive and negative, respectively. N/D, Not detected due to their sensitivity to trypsin.

^*Cell lines have been tested for the susceptibility to SADS-CoV by Yang et al. (2019).

^#The primary cell line established in our laboratory.