Figure 2.

Pro-inflammatory effects of Ent on models of human intestinal epithelia. (a) HT29, DLD-1 and Caco-2/BBe cell monolayers were incubated with Ent (0–25 µM) for 24 h in serum-free media supplemented with 1% penicillin-streptomycin and culture supernatants were collected and assessed for IL-8 secretion by ELISA. (b) The monolayers of HT29, DLD-1, and Caco-2/BBe cells were incubated overnight with Ent (25 µM), FeCl₃ (Fe³⁺) or Ent with an equimolar concentration of FeCl₃ and supernatants were analyzed for IL-8. Con denotes cells treated with DMSO as vehicle control. In vitro assays were performed in triplicates and data represented as mean ± SEM. * p < .05, **p < .01, *** p < .001 and **** p < .0001