Figure 1.

The expression of PDF1.2a was highly induced upon Ctro invasion. a. RT–qPCR analysis of PDF1.2a in Arabidopsis plants inoculated with Ctro. A conidial suspension from Ctro (5 × 10⁵/mL with 0.1% glucose) was spray-inoculated onto 4- to 5-week-old Arabidopsis plants. The leaf samples were collected at 6- and 17-hours post-inoculation (hpi). As a control, 0.1% glucose without Ctro was sprayed onto the plants and the leaf samples were collected at 17 h after this ‘mock’ treatment. Total RNA was extracted using PureLink RNA Mini kits (Thermo Fisher Scientific, Waltham, MA, USA) and treated with DNase. Takara Prime Script™ RT kits (Takara Bio Inc., Shiga, Japan) was used for the cDNA synthesis. Takara TB Green™ Premix Ex Taq™ I was used for RT–qPCR with the primers listed in Supplementary Table 1. Arabidopsis UBC21 (At5g25760) was used as an internal control for normalizing the level of cDNA.¹⁴ RT–qPCR analysis was performed using a Thermal Cycler Dice Real Time System TP810 (Takara Bio Inc.). Means and standard deviations (SDs) were derived from three independent samples. b. The expression of PDF1.2a was induced at 6 hpi compared with the mock treatment. The RT–qPCR data on mock and Ctro 6 hpi are shown with a different scale on the Y-axis in Figure 1a. The statistical significance of differences in gene expression level was determined by Tukey’s honestly significant difference (HSD) test (P < .01). The experiment was repeated twice, with similar results