Figure 3.

Both EDR1 and ORA59 are required for Ab-invasion triggered expression of PDF1.2a but not of PAD3. a. The invasion of Ab strongly induced the expression of PDF1.2a, which depended on EDR1 and ORA59. A suspension of Ab conidia (5 × 10⁵/mL) was spray-inoculated onto 4- to 5-week-old Arabidopsis plants, and inoculated leaves were collected at 0, 4, 12, 24, 48, 72 and 96 hpi. Subsequent RT–qPCR analysis was performed as described in Figure 1. The means and SDs were derived from three independent experiments. b. EDR1 and ORA59 are dispensable for Ab invasion-triggered expression of PAD3. Primers used for the RT–qPCR of PAD3 transcripts are listed in Supplementary Table 1. The means and SDs were derived from three independent samples. The experiment was repeated twice, with similar results