Figure 4.
ORA59 contributes to postinvasive resistance against Ab. a. Quantitative analysis of lesion development caused by Ab. A suspension of Ab conidia (5 × 105/mL) was drop-inoculated onto mature leaves of 4–5-week-old Arabidopsis plants. At 4 dpi, lesion development was measured. The means and SDs were calculated from three independent experiments. For the statistical analysis, lesion development in ora59 was compared with WT Col-0 using two-tailed Student’s t tests (*P < .05). b. Quantitative analysis of invasion ratio by Ab. A conidial suspension (1 × 105/mL) of Ab was drop-inoculated onto 4 to 5-week-old plants and kept at 100% humidity. The inoculated leaves were collected at 12 hpi, and then subjected to trypan blue staining assays. The presence or absence of invasive hyphae from at least 50 germinating conidia were counted in each experiment. The means and SDs were calculated from three independent experiments. Statistical comparisons of the Ab invasion ratios on WT Col-0 and tested mutants were conducted using two-tailed Student’s t tests. The invasion ratio of the ora59 mutants was not significantly different from that of WT (Col-0)