Figure 2.

H₂S enhances the homing potential of HSPCs. (a) Graphical representation of experimental setup for evaluating homing of different subsets of HSPCs. 3 × 10⁶ murine BMMNCs were treated with vehicle/NaHS, labeled with CFSE dye, washed and transplanted into lethally irradiated host mice. After 16 hrs, the host mice were sacrificed, BMMNCs isolated, lineage depleted and stained with surface markers and subsequently analyzed through flow cytometry. (b) This panel shows an increase in the homed CFSE⁺ LSK cells after NaHS treatment (n = 4 mice/group). (c) LSK population was analyzed further by staining with CD34 surface marker and CFSE⁺ LSK cells which were CD34⁺ (ST-HSCs) did not show a significant increase in the homed cells after treatment whereas, (d) a significant increase in the number of CD34⁻ (LT-HSCs) homed events were observed after NaHS treatment. Data are expressed as mean ± SEM for n = 4 mice, each assayed individually