Table 4. Equilibrium Dimer Fraction and Concentration as a Function of Substrate-Independent and -Dependent Monomer Concentrations in Multiples of Kda.
| Kd (μM) | [monomer] (μM) | dimer fraction | [dimer] (μM) | monomer fraction | [monomer] (μM) |
|---|---|---|---|---|---|
| Unbound | |||||
| 5.0 | 1·Kd = 5.0 | 0.5 | 2.5 | 0.5 | 2.5 |
| 5.0 | 2·Kd = 10.0 | 0.67 | 6.7 | 0.33 | 3.3 |
| 5.0 | 3·Kd = 15.0 | 0.75 | 11.25 | 0.25 | 3.75 |
| 5.0 | 10·Kd = 50.0 | 0.91 | 45.5 | 0.09 | 4.5 |
| Substrate-Bound | |||||
| 0.8 | 1·Kd = 0.8 | 0.5 | 0.4 | 0.5 | 0.4 |
| 0.8 | 2·Kd = 1.6 | 0.67 | 1.07 | 0.33 | 0.53 |
| 0.8 | 3·Kd = 2.4 | 0.75 | 1.8 | 0.25 | 0.6 |
| 0.8 | 10·Kd = 8.0 | 0.91 | 7.28 | 0.09 | 0.72 |
a
Based on the Hill approximation.