Figure 3.

ISnorm detects drastic transcriptomic changes in the Deng dataset of embryogenesis. (A) Effective library size estimated by ISnorm, Scran and DEseq2. (B) Number of genes detected as DE genes using DESeq2 by feeding size factors from ISnorm and other existing methods. (C) Number of maternal genes with log₂ fold change >0 and 0.05 < FDR < 0.15 (light red) and number of maternal genes with log₂ fold change >0 and FDR < 0.05 (dark red) inferred by feeding DESeq2 with size factors from ISnorm and other existing methods.