Table 2.

Error rates as obtained from raw read alignments against E. coli K12 DH5α (ONT reads) and K12 MG1655 (PacBio reads)

Dataset	Alignment tool	Aligned reads [%]	Substi-tutions [%]	Insertions [%]	Deletions [%]	Total error [%]	Longest perfect match [bp]	Longest alignment [bp]
ONT	blasr	99.76	3.50	4.01	4.94	12.45	369	99 183
ONT	graphmap	99.72	4.38	3.94	4.76	13.08	373	142 108
ONT	last	100.00	5.12	3.23	4.00	12.35	373	98 422
ONT	minimap2	99.83	4.33	3.59	4.47	12.39	371	98 780
ONT	average	99.83	4.33	3.69	4.54	12.56	372	109 623
PacBio	blasr	98.35	1.07	8.33	3.43	12.83	191	42 971
PacBio	graphmap	98.18	1.81	8.82	3.32	13.95	189	49 318
PacBio	last	100.00	2.07	7.40	2.89	12.36	192	42 468
PacBio	minimap2	98.01	1.75	7.60	2.98	12.33	189	42 737
PacBio	average	98.89	1.68	8.04	3.16	12.88	190	44 373