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The schematic diagram showing the final multiepitope vaccine protein. The 657-amino acid designed vaccine consists of adjuvant (brown) and HTL (green) epitopes linked by the EAAK linker (black) at the N-terminal end of the protein, where GPGPG (orange) and AAY (purple) linkers were used to join the HTL (green) and CTL (blue) epitopes, respectively. A 6×His (black epitopes) tag is added to the C-terminal end of vaccine protein for identification and purification.
